Synonyms:
ANGPT1, AGP-1, ANG-1, AGPT, angiopoietin 1 isoform a, angiopoietin 1 isoform b, KIAA0003.
Description:
Angiopoietin-1 (Ang-1) Human, a 66 kDa protein consisting of 476 amino acid residues (N21-F496) is fused to an N-terminal myc-tag and produced in insect cells.
Source:
Insect Cells
Physical Appearance:
Sterile Filtered clear liquid formulation.
Formulation:
The Angiopoietin 1 (0.111mg/ml) contains 50mM Tris-HCL, pH 7.5, 150mM NaCl, 0.05% CHAPS.
Stability:
ANG-1 although stable at 14°C 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The biological activity was determined by the induction of endothelial cell sprouting as described in Korff et al., 2001.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.27 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of ANG-1 as a Reference Standard.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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