Synonyms:
VGR, VGR1, BMP-6.
Description:
Bone Morphogenetic Protein-6 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 142 amino acids and having a molecular mass of 15 kDa.
3 additional amino acids were added from the N-terminal Ala-Pro-Thr to increase bacterial expression.
The BMP-6 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Solubility:
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-6 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized BMP 6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bone Morphogenetic Protein 6 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser-Ala-Ser-Ser-Arg-Arg.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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