Synonyms:
CTF1, CT1, CT-1, Cardiophin 1.
Description:
The Cardiotrophin His-Tagged Fusion Protein Human, produced in E. coli, is 22.5 kDa protein containing 200 amino acid residues of the human Cardiotrophin and 12 additional amino acid residues – His Tag (underlined).
Specificity:
The amino acid sequence of the cardiotrophin 1 human recombinant is 100% Homologous to the amino acid sequence of the cardiotrophin-1 human.
Purification Method:
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Source:
Escherichia Coli.
Purity:
Purity of CTF1 Human Recombinant is greater than 90% (SDS-PAGE analyzed).
Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Solubility:
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized Cardiophin 1 remains stable until the expiry date when stored at -20°C.
Applications:
Western blotting.
Amino acid sequence:
MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.
References:
- Freed DH, Moon MC, Borowiec AM, Jones SC, Zahradka P, Dixon IM. CT-1 expression in experimental myocardial infarction and potential role in post-MI wound healing. Mol Cell Biochem. 2003 Dec; 254(1-2): 247-56.
- Freed DH, Borowiec AM, Angelovska T, Dixon IM. Induction of protein synthesis in cardiac fibroblasts by cardiotrophin-1: integration of multiple signaling pathways. Cardiovasc Res. 2003 Nov 1; 60(2): 365-75.
- Bristow MR, Long CS. Cardiotrophin-1 in heart failure. Circulation. 2002 Sep 17; 106(12): 1430-2.
- Liao Z, Brar BK, Cai Q, Stephanou A, O'Leary RM, Pennica D, Yellon DM, Latchman DS. Cardiotrophin-1 (CT-1) can protect the adult heart from injury when added both prior to ischaemia and at reperfusion. Cardiovasc Res. 2002 Mar; 53(4): 902-10.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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