Synonyms:
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903.
Description:
The Clusterin Human contains a total 454 amino acids and a calculated molecular mass of 53058Da. The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal His tag 27AA (bold).
Source:
293 cell line (Human embryonic kidney).
Purification Method:
Ni-NTA chromatography.
Formulation:
Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
Solubility:
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C.
Purity:
Greater than 95% (SDS PAGE analyzed).
Amino acid sequence:
DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEGSL GSGWSHPQFE KTGHHHHHHH HGGQ
Refrences:
- Trougakos IP, Gonos ES. Functional analysis of clusterin/apolipoprotein J in cellular death induced by severe genotoxic stress. Ann N Y Acad Sci. 2004 Jun;1019: 206-10.
- Carver JA, Rekas A, Thorn DC, Wilson MR. Small heat-shock proteins and clusterin: intra- and extracellular molecular chaperones with a common mechanism of action and function? IUBMB Life. 2003 Dec; 55(12): 661-8. Review.
- Min BH, Kim BM, Lee SH, Kang SW, Bendayan M, Park IS. Clusterin expression in the early process of pancreas regeneration in the pancreatectomized rat. J Histochem Cytochem. 2003 Oct; 51(10): 1355-65.
- Park JH, Park JS, Ju SK, Lee KB, Park YK, Kang MH, Na SY, You KH. Clusterin mRNA expression in apoptotic and activated rat thymocytes. Cell Res. 2003 Feb; 13(1): 49-58.
- Jones SE, Jomary C. Clusterin. Int J Biochem Cell Biol. 2002 May; 34(5): 427-31. Review.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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