Synonyms:
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, APO-J.
Description:
The Clusterin Rat was constructed as a recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Clusterin Rat His-Tagged Fusion Protein, produced in E.coli, is 26.5 kDa protein containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues – His-Tag, T7-Tag (underlined).
Source:
Escherichia Coli.
Purification Method:
Ni-NTA chromatography.
Specificity:
The amino acid sequence of the Clusterin Rat recombinant is 100% homologous to the amino acid sequence 146-360 of the Clusterin Rat precursor.
Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris pH 7.2.
Solubility:
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C.
Purity:
Greater than 95% (SDS PAGE analyzed).
Applications:
Western blotting
Amino acid sequence:
ASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEPQDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFPDVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQYNELLHSLQS KMLNTSSLLE QALEHHHHHH
References:
- Trougakos IP, Gonos ES. Functional analysis of clusterin/apolipoprotein J in cellular death induced by severe genotoxic stress.Ann N Y Acad Sci. 2004 Jun;1019:206-10.
- Carver JA, Rekas A, Thorn DC, Wilson MR. Small heat-shock proteins and clusterin: intra- and extracellular molecular chaperones with a common mechanism of action and function? IUBMB Life. 2003 Dec; 55(12): 661-8. Review.
- Min BH, Kim BM, Lee SH, Kang SW, Bendayan M, Park IS. Clusterin expression in the early process of pancreas regeneration in the pancreatectomized rat. J Histochem Cytochem. 2003 Oct; 51(10): 1355-65.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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