Synonyms:
Tumor-derived hypophosphatemia-inducing factor, HYPF, ADHR, HPDR2, PHPTC, FGF23, FGF-23, Fibroblast Growth Factor-23.
Description:
Fibroblast Growth Factor-23 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 243 amino acids and having a molecular mass of 28 kDa.
The FGF-23 is purified by chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered white lyophilized powder.
Formulation:
The protein (0.5mg/ml) was lyophilized from 25mM Tris pH7.5 and 0.6M NaCl solution.
Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-23 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Fibroblast Growth Factor 23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-23 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
MLGARLRLW VCALCSVCSM SVLRAYPNAS PLLGSSWGG
NSYHLQIHKN GHVDGAPHQT IYSALMIRSE DAGFVVITGV MSRRYLCMDF RGNIFGSHYF DPENCRFQHQ TLENGYDVYH SPQYHFLVS LGRAKRAFLP GMNPPPYSQF LSRRNEIPLI HFNTPIPRRH TRSAEDDSER DPLNVLKPRA RMTPAPASCS QELPSAEDNS PMASDPLGVV RGGRVNTHAG GTGPEGCRPF AKI
Biological Activity:
Treatment with hrFGF23 has been shown to induce FGFR mediated Erk phosphorylation, reduce plasma PTH levels in rats and to reduce blood phosphate levels (Ref 1).
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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