Synonyms:
Fibroblast growth factor 22, FGF-22.
Description:
Fibroblast Growth Factor-22 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17173 Dalton.
The FGF-22 is purified by chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered white lyophilized powder.
Formulation:
The sterile protein powder is lyophilized with no additives.
Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-22 sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Fibroblast Growth Factor 22 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-22 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Ser-Ala-Ser.
Biological Activity:
Measured in a cell proliferation assay using IEC6 cell line .
(20 ng/ml can stimulate the cell proliferation of IEC6 cell line).
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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