Synonyms:
Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14.
Description:
Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8 Dalton.
To enable bacterial expression of GDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys.
GDF5 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized without any additives.
Solubility:
It is recommended to reconstitute the lyophilized Growth Differentiation Factor 5 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly.
Biological Activity:
Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.
Protein content:
Protein quantitation was carried out by two independent methods:
UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Analysis by RP-HPLC, using a standard solution of GDF 5 as a Reference Standard.
Usage:
ProteinTools products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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